Smad3 (4H4) Mouse mAb - diagbio | 戴格生物

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Smad3 (4H4) Mouse mAb

db6613

Product Name : Smad3 (4H4) Mouse mAb

Cat.No.: db6613

Synonyms : hMAD3; HSPC193; LDS1C; MADH3; JV15 2

Application : WB, IHC-P

Reactivity : Human, Rat, Mouse

Host species : Mouse

Background Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Immunogen Purified recombinant protein expressed in E.coli
Gene ID 4088
Swiss Prot P84022
Synonyms hMAD3; HSPC193; LDS1C; MADH3; JV15 2
Reactivity Human, Rat, Mouse
Application WB, IHC-P
Recommended dilution WB: 1:500-1:1000
IHC: 1:50-1:100
Calculated MW 48 kDa
Observed MW 48 kDa
Host species Mouse
Clonality Monoclonal
Clonality No. 4H4-10A10-7B4
Isotype IgG1
Purity Affinity Purification
Conjugation Un-conjugated
Storage/Stability Store at -20°C. Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Stable for 12 months from date of receipt.

Western blot analysis of Smad3 (4H4)in 293T, Jurkat, rat Heart, mouse Heart lysates using Smad3 antibody.
Immunohistochemistry analysis of paraffin-embedded Human Liver Tissue using Smad3 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Immunohistochemistry analysis of paraffin-embedded Human Colon Carcinoma using Smad3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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