PI3-Kinase p85 alpha (1C8) Mouse mAb - diagbio | 戴格生物

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PI3-Kinase p85 alpha (1C8) Mouse mAb

db6611

Product Name : PI3-Kinase p85 alpha (1C8) Mouse mAb

Cat.No.: db6611

Synonyms : GRB1; p85 alpha; PI3K; PIK3R1

Application : WB, IHC-P

Reactivity : Human, Mouse, Rat

Host species : Mouse

Background Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed/17626883, PubMed/19805105, PubMed/7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed/20348923).
Immunogen Purified recombinant protein expressed in E.coli
Gene ID 5295
Swiss Prot P27986
Synonyms GRB1; p85 alpha; PI3K; PIK3R1
Reactivity Human, Mouse, Rat
Application WB, IHC-P
Recommended dilution WB: 1:500-1:1000
IHC: 1:50-1:100
Calculated MW 84 kDa
Observed MW 84 kDa
Host species Mouse
Clonality Monoclonal
Clonality No. 1C8-2H4-9D9
Isotype IgG1
Purity Affinity Purification
Conjugation Un-conjugated
Storage/Stability Store at -20°C. Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Stable for 12 months from date of receipt.

Immunohistochemistry analysis of paraffin-embedded rat Liver Tissue using PI3 Kinase p85 alpha (1C8) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Immunohistochemical analysis of paraffin-embedded Human tonsils using PI3 Kinase p85 alpha (1C8) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Western blot analysis of PI3 Kinase p85 alpha (1C8) in Jurkat, rat Brain, Raw264.7, Ramos, Raji lysates using PI3 Kinase p85 alpha (1C8) antibody
Western blot analysis of PI3 Kinase p85 alpha (1C8) in Hela, A549, HL-60, U2OS, C6 lysates using PI3 Kinase p85 alpha (1C8) antibody.

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