PI3-Kinase p85 alpha (3H5) Mouse mAb
Product Name :
PI3-Kinase p85 alpha (3H5) Mouse mAb
Cat.No.:
db6610
Synonyms :
GRB1; p85 alpha; PI3K; PIK3R1
Application :
WB, IHC-P
Reactivity :
Human, Mouse, Rat
Host species :
Mouse
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Background
Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed/17626883, PubMed/19805105, PubMed/7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed/20348923).
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Immunogen
Purified recombinant protein expressed in E.coli
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Gene ID
5295
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Swiss Prot
P27986
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Synonyms
GRB1; p85 alpha; PI3K; PIK3R1
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Reactivity
Human, Mouse, Rat
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Application
WB, IHC-P
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Recommended dilution
WB: 1:500-1:1000
IHC: 1:50-1:100
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Calculated MW
84 kDa
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Observed MW
84 kDa
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Host species
Mouse
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Clonality
Monoclonal
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Clonality No.
3H5-1H4-9D2
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Isotype
IgG1
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Purity
Affinity Purification
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Conjugation
Un-conjugated
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Storage/Stability
Store at -20°C. Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Stable for 12 months from date of receipt.
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Immunohistochemistry analysis of paraffin-embedded rat Liver Tissue using PI3 Kinase p85 alpha (3H5) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Immunohistochemical analysis of paraffin-embedded Human tonsils using PI3 Kinase p85 alpha (3H5) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Western blot analysis of PI3 Kinase p85 alpha (3H5) in THP-1, MCF-7, Goat Muscle, CHO-K1, C6, 3T3 lysates using PI3 Kinase p85 alpha (3H5) antibody
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Western blot analysis of PI3 Kinase p85 alpha (3H5) in Hela, A549, HL-60, U2OS, C6 lysates using PI3 Kinase p85 alpha (3H5) antibody.
订购信息
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Package
Price (RMB)
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50μL
1398
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100μL
2398
- 货期:现货
For research use only. Not intended for diagnostic.