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Hsp90 alpha (5D6) Mouse mAb
db6605
Datasheet
- Background Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function (PubMed/11274138, PubMed/15577939, PubMed/15937123, PubMed/27353360). Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle (PubMed/27295069, PubMed/26991466). Apart from its chaperone activity, it also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription at least at three different levels. In the first place, they alter the steady-state levels of certain transcription factors in response to various physiological cues. Second, they modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases, and thereby respond to the change in the environment. Third, they participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression (PubMed/25973397). Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes (PubMed/11276205). Antagonizes STUB1-mediated inhibition of TGF-beta signaling via inhibition of STUB1-mediated SMAD3 ubiquitination and degradation (PubMed/24613385).
- Immunogen Purified recombinant protein expressed in E.coli
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Gene ID
3320
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Swiss Prot
P07900
- Synonyms EL52; Heat shock 86 KDa; HSP86; HSP90N; HSPC1
- Reactivity Human, Rat, Mouse
- Application WB, IHC-P
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Recommended dilution
WB: 1:500-1:1000
IHC: 1:50-1:100 - Calculated MW 85 kDa
- Observed MW 95 kDa
- Host species Mouse
- Clonality Monoclonal
- Clonality No. 5D6-2G8-6D5
- Isotype IgG1
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Stable for 12 months from date of receipt.
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Immunohistochemistry analysis of paraffin-embedded Human Colon Caricnoma using Hsp90 alpha (5D6) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. -
Western blot analysis of Hsp90 alpha (5D6) in K562, Ramos, Jurkat, rat Brain lysates using Hsp90 alpha (5D6) antibody -
Western blot analysis of Hsp90 alpha (5D6) in Hela, A549, HL6, U2OS, C6 lysates using Hsp90 alpha (5D6) antibody
订购信息
- Package Price (RMB)
- 50μL 1398
- 100μL 2398
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
