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JAK2 (6B4) Mouse mAb
db6585
Datasheet
- Background Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins (PubMed/7615558). Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins (PubMed/9618263). Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. Part of a signaling cascade that is activated by increased cellular retinol and that leads to the activation of STAT5 (STAT5A or STAT5B) (PubMed/21368206). In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation (PubMed/20098430). Plays a role in cell cycle by phosphorylating CDKN1B (PubMed/21423214). Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin (PubMed/19783980).
- Immunogen Synthetic peptide conjugated to KLH
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Gene ID
3717
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Swiss Prot
O60674
- Synonyms JAK2; Tyrosine-protein kinase JAK2; Janus kinase 2; JAK-2
- Reactivity Human, Rat, Mouse
- Application IHC-P
- Recommended dilution IHC: 1:50-1:100
- Calculated MW 120 kDa
- Host species Mouse
- Clonality Monoclonal
- Clonality No. 6B4-8G2-1C4
- Isotype IgG1
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Stable for 12 months from date of receipt.
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Immunohistochemistry analysis of paraffin-embedded Human Breast Carcinoma Tissue using JAK2 (6B4) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. -
Immunohistochemical analysis of paraffin-embedded Human tonsils using JAK2 (6B4) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
订购信息
- Package Price (RMB)
- 50μL 1398
- 100μL 2398
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
