Product Name : Phospho-Smad3 (Ser425) (7H6) Mouse mAb
Cat.No.: db6578
Synonyms : SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
Application : IHC-P
Reactivity : Human, Rat, Mouse
Host species : Mouse
- Background Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
- Immunogen Synthetic peptide conjugated to KLH
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Gene ID
4088
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Swiss Prot
P84022
- Synonyms SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
- Reactivity Human, Rat, Mouse
- Application IHC-P
- Recommended dilution IHC: 1:50-1:100
- Calculated MW 50 kDa
- Host species Mouse
- Clonality Monoclonal
- Clonality No. 7H6-4E5-4H2
- Isotype IgG1
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Stable for 12 months from date of receipt.
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Immunohistochemistry analysis of paraffin-embedded Human Skin Tissue using Phospho-Smad3 (Ser425) (7H6) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. -
Immunohistochemical analysis of paraffin-embedded Human tonsils using Phospho-Smad3 (Ser425) (7H6) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
- Package Price (RMB)
- 50μL 1398
- 100μL 2398
- 货期：现货
