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DDIT3 (7G7) Mouse mAb
db6566
Datasheet
- Background Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription/ dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG). Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity. Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response.
- Immunogen Synthetic peptide conjugated to KLH
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Gene ID
1649
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Swiss Prot
P35638
- Synonyms GADD153; DDIT3;MGC4154
- Reactivity Human, Rat, Mouse
- Application WB, IHC-P, ICC/IF
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Recommended dilution
WB: 1:500-1:1000
IHC: 1:50-1:100
ICC/IF: 1:50-1:200 - Calculated MW 19 kDa
- Observed MW 27 kDa
- Host species Mouse
- Clonality Monoclonal
- Clonality No. 7G7-3B2-2F10
- Isotype IgG1
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Stable for 12 months from date of receipt.
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Immunohistochemistry analysis of paraffin-embedded Human stomach tissue using DDIT3 (7G7) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Negative control was used by secondary antibody only. -
Immunofluorescence analysis of DDIT3 (7G7) in mouse brain tissue using DDIT3 (7G7) antibody(red),and DAPI (blue). -
Immunohistochemistry analysis of paraffin-embedded Human Stomach Carcinoma Tissue using CHOP antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. -
Immunohistochemistry analysis of paraffin-embedded Human Pancreas Carcinoma Tissue using CHOP antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. -
Western blot analysis of DDIT3 in mouse Liver lysates using DDIT3 (7G7) antibody.
订购信息
- Package Price (RMB)
- 50μL 1398
- 100μL 2398
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
