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PCNA (9C9) Mouse mAb
db6498
Datasheet
- Background Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed/24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair/ Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
- Immunogen Synthetic Peptide of PCNA
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Gene ID
5111
-
Swiss Prot
P12004
- Synonyms Proliferating Cell Nuclear Antigen; DNA polymerase delta auxiliary protein; PCNAR
- Reactivity Human, Mouse, Rat
- Application WB, IHC-P
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Recommended dilution
WB: 1:5000-1:10000
IHC: 1/50-1:200 - Calculated MW 29 kDa
- Observed MW 36 kDa
- Host species Mouse
- Clonality Monoclonal
- Clonality No. 9C9-5B5-2A3
- Isotype IgG1
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Stable for 12 months from date of receipt.
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Immunohistochemistry analysis of paraffin-embedded rat small intestine using PCNA (9C9) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. -
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using PCNA (9C9) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. -
Western blot analysis of PCNA in T47D, 3T3 and COS7 lysates using PCNA antibody. -
Western blot analysis of PCNA in Jurkat, CHO-K1, 3T3, Hela lysates using PCNA (9C9) antibody. -
Western blot analysis of PCNA (9C9) in MOT4, Raw264.7, 293T, CEM lysates using PCNA (9C9) antibody.
订购信息
- Package Price (RMB)
- 50μL 1398
- 100μL 2398
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
