Product Name : Caspase-8 (4C2) Mouse mAb
Cat.No.: db6486
Synonyms : CASP8; MCH5; Caspase-8; CASP-8; Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog; MACH
Application : WB, ICC/IF, IHC-P
Reactivity : Human, Mouse, Rat
Host species : Mouse
- Background Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
- Immunogen Recombinant Protein of Caspase-8
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Gene ID
841
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Swiss Prot
Q14790
- Synonyms CASP8; MCH5; Caspase-8; CASP-8; Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog; MACH
- Reactivity Human, Mouse, Rat
- Application WB, ICC/IF, IHC-P
-
Recommended dilution
WB: 1:500-1:1000
IHC: 1:50-1:100
ICC/IF: 1:50-1:200 - Calculated MW 55 kDa
- Observed MW 43,57 kDa
- Host species Mouse
- Clonality Monoclonal
- Clonality No. 4C2-3G4-5G7
- Isotype IgG1
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Stable for 12 months from date of receipt.
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Western blot analysis of Caspase8 in K562, 3T3, MCF-7 and Jurkat lysates using Caspase8 antibody. -
Immunohistochemistry analysis of paraffin-embedded mouse Spleen Tissue using Caspase8 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. -
Western blot analysis of Caspase 8 (2G12) in THP-1, rat Brain, Hela lysates using Caspase8 (4C2) antibody. -
Western blot analysis of Caspase 8 (2G12) in Jurkat, MOLT4 lysates using Caspase 8 (2G12) antibody -
Immunocytochemistry analysis of Caspase8 (4C2) (green) in Hela using Caspase8 (4C2) antibody,and DAPI(blue).
- Package Price (RMB)
- 50μL 1398
- 100μL 2398
- 货期：现货
