Product Name : PARP1 (10C2) Mouse mAb
Cat.No.: db6467
Synonyms : PARP1; ADPRT; PPOL; Poly [ADP-ribose] polymerase 1; PARP-1; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD(+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1
Application : WB, IHC-P
Reactivity : Human, Mouse, Rat
Host species : Mouse
- Background Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks (PubMed/17177976, PubMed/18172500, PubMed/19344625, PubMed/19661379, PubMed/23230272). Mediates the poly(ADP-ribosyl)ation of APLF and CHFR (PubMed/17396150). Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production (PubMed/17177976). Required for PARP9 and DTX3L recruitment to DNA damage sites (PubMed/23230272). PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites (PubMed/23230272). Mediates serine ADP-ribosylation of target proteins following interaction with HPF1; HPF1 conferring serine specificity (PubMed/28190768). Mediates the poly(ADP-ribosyl)ation of histones in a HPF1-dependent manner (PubMed/27067600). Involved in the synthesis of ATP in the nucleus, together with NMNAT1, PARG and NUDT5 (PubMed/27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed/27257257).
- Immunogen Synthetic Peptide of Cleaved PARP
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Gene ID
142
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Swiss Prot
P09874
- Synonyms PARP1; ADPRT; PPOL; Poly [ADP-ribose] polymerase 1; PARP-1; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD(+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1
- Reactivity Human, Mouse, Rat
- Application WB, IHC-P
-
Recommended dilution
WB: 1:500-1:1000
IHC: 1:50-1:100 - Calculated MW 113 kDa
- Observed MW 116 kDa
- Host species Mouse
- Clonality Monoclonal
- Clonality No. 10C2-1A2-8F8
- Isotype IgG1
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Stable for 12 months from date of receipt.
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Western blot analysis of PARP1 (10C2) in Hela, MCF-7 and Jurkat lysates using PARP (10C2) antibody. -
Immunohistochemistry analysis of paraffin-embedded Human Tonsil Tissue using Cleaved PARP antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. -
Western blot analysis of PARP1 (10C2) in K562, Ramos, Jurkat lysates using PARP (1C2) antibody. -
Western blot analysis of PARP1 (10C2) in 3T3, Hela lysates using PARP1 (10C2) antibody.
- Package Price (RMB)
- 50μL 1398
- 100μL 2398
- 货期：现货
