DiMethyl-Histone H3 (Lys27) (5F6) Mouse mAb - diagbio | 戴格生物

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DiMethyl-Histone H3 (Lys27) (5F6) Mouse mAb

db6450

Product Name : DiMethyl-Histone H3 (Lys27) (5F6) Mouse mAb

Cat.No.: db6450

Synonyms : H3K27me2; H3 histone; HIST1H3A; Histone cluster 1; H3a

Application : WB, IP, ICC/IF

Reactivity : Human, Mouse, Rat

Host species : Mouse

Background Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.MiscellaneousThis histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2).
Immunogen Synthetic Peptide of Histone H3 (Di Methyl Lys27)
Gene ID 8350
Swiss Prot P68431
Synonyms H3K27me2; H3 histone; HIST1H3A; Histone cluster 1; H3a
Reactivity Human, Mouse, Rat
Application WB, IP, ICC/IF
Recommended dilution WB: 1:500-1:1000
ICC/IF: 1:50-1:200
IP: 1:20
Calculated MW 15 kDa
Observed MW 15 kDa
Host species Mouse
Clonality Monoclonal
Clonality No. 5F6-6G6-6F9
Isotype IgG1
Purity Affinity Purification
Conjugation Un-conjugated
Storage/Stability Store at -20°C. Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Stable for 12 months from date of receipt.

Immunoprecipitation analysis of DiMethyl-Histone H3 (Lys27) (5F6) in Hela lysates using DiMethyl-Histone H3 (Lys27) (5F6) antibody.
Western blot analysis of DiMethyl-Histone H3 (Lys27) (5F6) in C6, 3T3, C2C12 lysates using DiMethyl-Histone H3 (Lys27) (5F6) antibody
Immunocytochemistry analysis ofDiMethyl-Histone H3 (Lys27) in HeLa cells using DiMethyl-Histone H3 (Lys27) (5F6) antibody.
Western blot analysis of DiMethyl-Histone H3 (Lys27) (5F6) in Hela, rat heart, mouse heart, HUVEC, Jurkat lysates using DiMethyl-Histone H3 (Lys27) (5F6) antibody.

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