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NF-kB p65 (5A10) Mouse mAb
db6428
Datasheet
- Background NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed/15790681).
- Immunogen Synthetic Peptide of NFkB p65
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Gene ID
5970
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Swiss Prot
Q04206
- Synonyms NFKB3; RELA; TF65; Transcription factor p65; p65; NFkB
- Reactivity Human, Mouse, Rat
- Application WB, IHC-P, IP
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Recommended dilution
WB: 1:500-1000
IHC: 1:50-1:100
IP: 1:20 - Calculated MW 65 kDa
- Observed MW 65 kDa
- Host species Mouse
- Clonality Monoclonal
- Clonality No. 5A10-1F10-1D8
- Isotype IgG1
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Stable for 12 months from date of receipt.
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Western blot analysis of NFκB p65 in Jurkat, MCF-7, SKBR3, Hela and 3T3 lysates using NFκB p65 antibody. -
Immunoprecipitation analysis of NF-KB p65 in Hela lysates using NF-KB p65 (5A10) antibody -
Immunohistochemistry analysis of paraffin-embedded mouse hippocampus using NFκB p65 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. -
Western blot analysis of NF-KB p65 (5A10) in CHO-K1, rat brain, C6, 3T3, Hela lysates using NF-KB p65 (5A10) antibody
订购信息
- Package Price (RMB)
- 50μL 1398
- 100μL 2398
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
