MCM2 (2B5) Mouse mAb - diagbio | 戴格生物

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MCM2 (2B5) Mouse mAb

db6407

Product Name : MCM2 (2B5) Mouse mAb

Cat.No.: db6407

Synonyms : MCM2; BM28; CCNL1; CDCL1; KIAA0030; DNA replication licensing factor MCM2; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28

Application : WB, IHC-F, IHC-P, ICC/IF, FC, IP

Reactivity : Human, Mouse

Host species : Mouse

Background The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
Immunogen Purified recombinant fragment of human MCM2 expressed in E. Coli
Gene ID 4171
Swiss Prot P49736
Synonyms MCM2; BM28; CCNL1; CDCL1; KIAA0030; DNA replication licensing factor MCM2; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28
Reactivity Human, Mouse
Application WB, IHC-F, IHC-P, ICC/IF, FC, IP
Recommended dilution WB: 1:500-1:1000
IHC: 1:50-1:100
ICC/IF: 1:50-1:200
IP: 1:20
FC: 1:50-1:100
Calculated MW 102 kDa
Observed MW 125 kDa
Host species Mouse
Clonality Monoclonal
Clonality No. 2B5-H4-A9
Isotype IgG1
Purity Affinity Purification
Conjugation Un-conjugated
Storage/Stability Store at -20°C. Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Stable for 12 months from date of receipt.

Western blot analysis of MCM2 in Jurkat, K562, 3T3 and Hela lysates using MCM2 antibody.
Immunoprecipitation analysis of MCM2 (2B5) in Hela lysates using MCM2 antibody.
Immunofluorescence analysis of MCM2 (2B5) in Hela cells using MCM2 antibody(green),and DAPI (blue).
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using MCM2 (2B5) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Immunohistochemistry analysis of paraffin-embedded ovarian cancer tissues using MCM2 (2B5) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Immunohistochemistry analysis of paraffin-embedded colon cancer tissues using MCM2 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

订购信息

Package Price (RMB)
50μL 1398
100μL 2398
货期：现货