Product Name : AKT1/2/3 Mouse mAb
Cat.No.: db6344
Synonyms : AKT; AKT1; AKT1 kinase; AKT2; AKT2 kinase; Akt3; AKT3_HUMAN; CAKT; DKFZp434N0250; kinase Akt1; MGC99656; Murine thymoma viral (v-akt) homolog 2; PKB; PKB gamma; PKB-GAMMA; PKB/Akt; PKBALPHA; PKBB; PKBBETA; PKBG; PKBGAMMA; PRKBA; PRKBB; PRKBG; Protein kinase Akt-3; Protein kinase B; Protein kinase B gamma; RAC; rac protein kinase alpha; rac protein kinase beta; RAC-gamma; RAC-gamma serine/threonine-protein kinase; RAC-PK-gamma; RACALPHA; RACalpha serine/threonine kinase; RACBETA; RACgamma; RACgamma serine/threonine protein kinase; RACPKgamma; serine threonine protein kinase; STK-2; STK2; thymoma viral proto oncogene 1; thymoma viral proto oncogene; vakt murine thymoma viral oncogene homolog 1; vakt murine thymoma viral oncogene homolog 2; vakt murine thymoma viral oncogene homolog 3
Application : WB, IP
Reactivity : Human, Mouse, Rat
Host species : Mouse
- Background Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.
- Immunogen Purified recombinant human AKT1 protein fragments expressed in E.coli.AKT1 interacts (via the C-terminus) with CCDC88A (via its C-terminus). Interacts with GRB10; the interaction leads to GRB10 phosphorylation thus promoting YWHAE-binding By similarity. Interacts with AGAP2 (isoform 2/PIKE-A); the interaction occurs in the presence of guanine nucleotides. Interacts with AKTIP. Interacts (via PH domain) with MTCP1, TCL1A AND TCL1B. Interacts with CDKN1B; the interaction phosphorylates CDKN1B promoting 14-3-3 binding and cell-cycle progression. Interacts with MAP3K5 and TRAF6. Interacts with BAD, PPP2R5B, STK3 and STK4. Interacts (via PH domain) with SIRT1. Interacts with SRPK2 in a phosphorylation-dependent manner. Interacts with RAF1. Interacts with TRIM13; the interaction ubiquitinates AKT1 leading to its proteasomal degradation. Interacts with TNK2 and CLK2. Interacts (via the C-terminus) with THEM4 (via its C-terminus). Interacts with and phosphorylated by PDPK1.AKT2 interacts (via PH domain) with MTCP1, TCL1A AND TCL1B. Interacts with CLK2, PBH2 and TRAF6. AKT3 interacts (via PH domain) with TCL1A; this enhances AKT3 phosphorylation and activation. Interacts with TRAF6
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Gene ID
207
208
10000
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Swiss Prot
P31749
P31751
Q9Y243
- Synonyms AKT; AKT1; AKT1 kinase; AKT2; AKT2 kinase; Akt3; AKT3_HUMAN; CAKT; DKFZp434N0250; kinase Akt1; MGC99656; Murine thymoma viral (v-akt) homolog 2; PKB; PKB gamma; PKB-GAMMA; PKB/Akt; PKBALPHA; PKBB; PKBBETA; PKBG; PKBGAMMA; PRKBA; PRKBB; PRKBG; Protein kinase Akt-3; Protein kinase B; Protein kinase B gamma; RAC; rac protein kinase alpha; rac protein kinase beta; RAC-gamma; RAC-gamma serine/threonine-protein kinase; RAC-PK-gamma; RACALPHA; RACalpha serine/threonine kinase; RACBETA; RACgamma; RACgamma serine/threonine protein kinase; RACPKgamma; serine threonine protein kinase; STK-2; STK2; thymoma viral proto oncogene 1; thymoma viral proto oncogene; vakt murine thymoma viral oncogene homolog 1; vakt murine thymoma viral oncogene homolog 2; vakt murine thymoma viral oncogene homolog 3
- Reactivity Human, Mouse, Rat
- Application WB, IP
- Recommended dilution WB: 1:1000
- Calculated MW 56 kDa
- Observed MW 60 kDa
- Host species Mouse
- Clonality Monoclonal
- Clonality No. 3B11-G8-B1
- Isotype IgG1
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Stable for 12 months from date of receipt.
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Western blot analysis of total AKT in 3T3, 293T, PC-12 and K562 lysates using AKT(pan) antibody. -
Immunoprecipitation analysis of AKT in Hela lysates using AKT(pan) antibody. -
Western blot analysis of AKT1, AKT2, AKT3 and DSBA recombinant antigen using DSBA antibody,and(Right)AKT1, AKT2 and AKT3 recombinant antigen fragments using AKT(pan) antibody.
- Package Price (RMB)
- 50μL 1398
- 100μL 2398
- 货期：现货
