Vimentin (4F8) Mouse mAb - diagbio | 戴格生物

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Vimentin (4F8) Mouse mAb

db6271

Product Name : Vimentin (4F8) Mouse mAb

Cat.No.: db6271

Synonyms : HEL113; CTRCT30; VIM; vimentin

Application : WB, ICC/IF, IP, IHC-F, IHC-P

Reactivity : Human

Host species : Mouse

Background Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Immunogen Purified recombinant human Vimentin protein fragments expressed in E.coli
Gene ID 7431
Swiss Prot P08670
Synonyms HEL113; CTRCT30; VIM; vimentin
Reactivity Human
Application WB, ICC/IF, IP, IHC-F, IHC-P
Recommended dilution WB: 1:500-1:1000
IHC: 1:50-1:100
ICC/IF: 1:50-1:200
IP: 1:20
Calculated MW 54 kDa
Observed MW 57 kDa
Host species Mouse
Clonality Monoclonal
Clonality No. 4F8-A5-H5
Isotype IgG2a
Purity Affinity Purification
Conjugation Un-conjugated
Storage/Stability Store at -20°C. Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Stable for 12 months from date of receipt.

Western blot analysis of Vimentin in Molt4, K562, COS7, Jurkat, Hela and Vimentin negative (Daudi, Ramos, Raji) lysates using Vimentin antibody.
Immunocytochemistry analysis of Vimentin (4F8) in Hela using Vimentin antibody.
Immunoprecipitation analysis of Vimentin (4F8) in Hela lysates using Vimentin antibody.
Immunohistochemistry analysis of paraffin-embedded Human tonsils using Vimentin (4F8) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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