Product Name : PCNA (2E1) Mouse mAb
Cat.No.: db6199
Synonyms : Cyclin;DNA polymerase delta auxiliary protein;HGCN8729;MGC8367;Mutagen-sensitive 209 protein;OTTHUMP00000030189;OTTHUMP00000030190;PCNA;Pcna/cyclin;PCNA_HUMAN;PCNAR;Polymerase delta accessory protein;Proliferating Cell Nuclear Antigen
Application : WB, IP, ICC/IF, IHC-F, IHC-P
Reactivity : Human, Mouse, Rat, Monkey, Hamster
Host species : Mouse
- Background Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase,but not apurinic-apyrimidinic (AP) endonuclease,APEX2 activities.Has to be loaded onto DNA in order to be able to stimulate APEX2.Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways.Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair/Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases,while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
- Immunogen Purified recombinant human PCNA protein fragments expressed in E.coli
-
Gene ID
5111
-
Swiss Prot
P12004
- Synonyms Cyclin;DNA polymerase delta auxiliary protein;HGCN8729;MGC8367;Mutagen-sensitive 209 protein;OTTHUMP00000030189;OTTHUMP00000030190;PCNA;Pcna/cyclin;PCNA_HUMAN;PCNAR;Polymerase delta accessory protein;Proliferating Cell Nuclear Antigen
- Reactivity Human, Mouse, Rat, Monkey, Hamster
- Application WB, IP, ICC/IF, IHC-F, IHC-P
-
Recommended dilution
WB: 1:1000
IHC: 1:50-1:200
ICC/IF: 1:100-1:200
IP: 1:20 - Calculated MW 29 kDa
- Observed MW 36 kDa
- Host species Mouse
- Clonality Monoclonal
- Clonality No. 2E1-G10-H10
- Isotype IgG2b
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Stable for 12 months from date of receipt.
-
Western blot analysis of PCNA in Hela, 3T3, COS7, C6 and CHO-K1 lysates using PCNA antibody. -
Immunocytochemistry analysis of PCNA in HeLa cells using PCNA antibody. -
Immunoprecipitation analysis of PCNA (2E1) in Hela lysates using PCNA antibody. -
Immunohistochemistry analysis of paraffin-embedded Human colorectal carcinoma using PCNA antibody (2E1G10H10).High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
- Package Price (RMB)
- 50μL 1398
- 100μL 2398
- 货期：现货
