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Aurora A (2E3) Mouse mAb
db6083
Datasheet
- Background Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizating p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis.
- Immunogen Purified recombinant human Aurora Kinase A protein fragments expressed in E.coli
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Gene ID
6790
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Swiss Prot
O14965
- Synonyms AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; AURORA2; PPP1R47
- Reactivity Human, Monkey
- Application WB, ICC/IF
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Recommended dilution
WB: 1:500-1:1000
ICC/IF: 1:50-1:200 - Calculated MW 46 kDa
- Observed MW 46 kDa
- Host species Mouse
- Clonality Monoclonal
- Clonality No. 2E3-C10-C11-G6
- Isotype IgG1
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Stable for 12 months from date of receipt.
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Western blot analysis of Aurora Kinase A in SW480 and COS7 lysates using Aurora Kinase A antibody. -
Immunocytochemistry analysis of Aurora A (2E3) in HeLa using Aurora Kinase A antibody. -
Immunofluorescence analysis of Aurora A (2E3) in Hela cells using Aurora Kinase A antibody(green) and Histone H3.1 (Phospho-Ser1) antibody (red),and DAPI (blue)
订购信息
- Package Price (RMB)
- 50μL 1398
- 100μL 2398
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
