Product Name : GRP78 BiP (9E4) Mouse mAb
Cat.No.: db6047
Synonyms : HSPA5; GRP78; 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP
Application : WB, ICC/IF, IHC-F, IHC-P
Reactivity : Human, Rat
Host species : Mouse
- Background When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) (PubMed 8020977) pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).
- Immunogen Purified recombinant human BiP/GRP78 (C-terminus) protein fragments expressed in E.coli
-
Gene ID
3309
-
Swiss Prot
P11021
- Synonyms HSPA5; GRP78; 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP
- Reactivity Human, Rat
- Application WB, ICC/IF, IHC-F, IHC-P
-
Recommended dilution
WB: 1:500-1:1000
IHC: 1:50-1:100
ICC/IF: 1:50-1:200 - Calculated MW 72 kDa
- Observed MW 78 kDa
- Host species Mouse
- Clonality Monoclonal
- Clonality No. 9E4-2A7-H6
- Isotype IgG1
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Stable for 12 months from date of receipt.
-
Western blot analysis of BiP/GRP78 (Cterminus) in Hela, C6, Lncap and MDA-MB-468 lysates using BiP/GRP78 (Cterminus) antibody. -
Immunocytochemistry analysis of GRP78 BiP (9E4) in HeLa using BiP/GRP78 (Cterminus) antibody. -
Immunohistochemistry analysis of paraffin-embedded Colorectal cancer using BiP/GRP78 (Cterminus) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
- Package Price (RMB)
- 50μL 1398
- 100μL 2398
- 货期：现货
