His Tag Rabbit pAb - diagbio | 戴格生物

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His Tag Rabbit pAb

db3296

Product Name : His Tag Rabbit pAb

Cat.No.: db3296

Application : WB, IHC, ICC/IF, FC, IP

Reactivity : Transfected

Host species : Rabbit

Background Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.
Immunogen A synthetic peptide of His tag
Reactivity Transfected
Application WB, IHC, ICC/IF, FC, IP
Recommended dilution WB: 1:1000
IHC: 1:500
ICC/IF: 1:20
FC: 1:500
IP: 1:20
Calculated MW 1 kDa
Observed MW According to the fused protein
Host species Rabbit
Clonality Polyclonal
Isotype IgG
Purity Affinity Purification
Conjugation Un-conjugated
Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

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