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CDKN2A/p14ARF Recombinant Rabbit mAb
db15315
Datasheet

Product Name : CDKN2A/p14ARF Recombinant Rabbit mAb

Cat.No.: db15315

Synonyms : ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A

Application : WB, ICC/IF, FC, IP

Reactivity : Human

Host species : Rabbit

  • Background This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq, Sep 2012]
  • Immunogen A synthetic peptide of human CDKN2A/p14ARF
  • Gene ID 1029
  • Swiss Prot Q8N726
  • Synonyms ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
  • Reactivity Human
  • Application WB, ICC/IF, FC, IP
  • Recommended dilution WB: 1:1000
    ICC/IF: 1:100
    FC: 1:20
    IP: 1:20
  • Calculated MW 14 kDa
  • Observed MW 14 kDa
  • Host species Rabbit
  • Clonality Monoclonal
  • Isotype IgG
  • Purity Affinity Purification
  • Conjugation Un-conjugated
  • Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
  • Western blot analysis of extracts from HeLa, MCF-7 cells using db15315 at 1:1000.
订购信息
  • Package Price (RMB)
  • 20μL 698
  • 50μL 1398
  • 100μL 2398
  • 货期:现货
For research use only. Not intended for diagnostic.