BackgroundThis gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011]
Storage/StabilityStore at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Western blot detection of Paxillin in C6 cell lysates using Paxillin antibody(1:1000 diluted).
Western blot analysis of extracts from HCT116 cells and Rat lung tissus using db14728 at 1:2000.
Immunohistochemical analysis of paraffin-embedded human breast cancer using db14728 antibody.
Immunofluorescent analysis of HeLa cells using db14728 antibody (green), and DAPI (blue).