BackgroundPhosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
ImmunogenA synthetic peptide of human PI 3 Kinase p85 alpha
Storage/StabilityStore at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Western blot detection of PI3 Kinase p85 alpha in Jurkat,C6,CHO-K1 cell lysates using PI3 Kinase p85 alpha antibody(1:1000 diluted).
Western blot analysis of extracts from Mouse muscle tissue using db13949 at 1:1000.
Western blot analysis of extracts from MCF-7 cells using db13949 at 1:1000.
Immunofluorescent analysis of MCF-7 cells using db13949 antibody (green), and DAPI (blue).