TriMethyl-Histone H3 (Lys27) (DGR14204) Rabbit mAb - diagbio

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Recombinant DGRmAb^®

TriMethyl-Histone H3 (Lys27) (DGR14204) Rabbit mAb

db13742

Datasheet

Product Name : TriMethyl-Histone H3 (Lys27) (DGR14204) Rabbit mAb

Cat.No.: db13742

Synonyms : H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FJ; H3C10; H3C11; HIST1H3J

Application : WB, IHC-P, ICC/IF

Reactivity : Human,Mouse,Rat

Host species : Rabbit

Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
Immunogen A synthetic methyl-peptide corresponding to residues surrounding Lys27 of human Histone H3
Gene ID 8356
Swiss Prot P68431
Synonyms H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FJ; H3C10; H3C11; HIST1H3J
Reactivity Human,Mouse,Rat
Application WB, IHC-P, ICC/IF
Recommended dilution WB: 1:1000
IHC-P: 1:200-1:500
ICC/IF: 1:500-1:1000
Calculated MW 15 kDa
Observed MW 17 kDa
Host species Rabbit
Clonality Monoclonal
Clonality No. DGR14204
Isotype IgG
Purity Affinity Purification
Conjugation Un-conjugated
Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Western blot detection of Histone H3 (tri methyl-Lys27) in C6,Jurkat cell lysates using Histone H3 (tri methyl-Lys27) antibody(1:1000 diluted).
Immunohistochemical analysis of paraffin-embedded human lung cancer using db13742 antibody.
Immunofluorescent analysis of HeLa cells using db13742 antibody (green), and DAPI (blue).

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