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TriMethyl-Histone H3 (Lys27) Recombinant Rabbit mAb
db13742
Datasheet
- Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
- Immunogen A synthetic methyl-peptide corresponding to residues surrounding Lys27 of human Histone H3
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Gene ID
8356
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Swiss Prot
P68431
- Synonyms H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FJ; H3C10; H3C11; HIST1H3J
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF
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Recommended dilution
WB: 1:1000
IHC-P: 1:200-1:500
ICC/IF: 1:500-1:1000 - Calculated MW 15 kDa
- Observed MW 17 kDa
- Host species Rabbit
- Clonality Monoclonal
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot detection of Histone H3 (tri methyl-Lys27) in C6,Jurkat cell lysates using Histone H3 (tri methyl-Lys27) antibody(1:1000 diluted). -
Immunohistochemical analysis of paraffin-embedded human lung cancer using db13742 antibody. -
Immunofluorescent analysis of HeLa cells using db13742 antibody (green), and DAPI (blue).
订购信息
- Package Price (RMB)
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
