BackgroundHistone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate.
ImmunogenA synthetic peptide of human KDM4B/JMJD2B
Storage/StabilityStore at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Western blot detection of KDM4B in 293,COS7 cell lysates using KDM4B/JMJD2B antibody(1:1000 diluted).
Western blot analysis of extracts from HeLa cells using db12931 at 1:1000.
Immunohistochemical analysis of paraffin-embedded human lung cancer using db12931 antibody.
Immunofluorescent analysis of HeLa cells using db12931 antibody (green), and DAPI (blue).