BackgroundThis gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]
Storage/StabilityStore at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Western blot detection of HDAC2 in K562,C6,3T3 cell lysates using HDAC2 antibody(1:1000 diluted).
Western blot analysis of extracts from C2C12 cells and Rat muscle tissue using db11911 at 1:1000.
Immunohistochemical analysis of paraffin-embedded human colon cancer using db11911 antibody.
Immunofluorescent analysis of HeLa cells using db11911 antibody (green), and DAPI (blue).