Phospho-Histone H3 (Thr3) Recombinant Rabbit mAb - diagbio | 戴格生物

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Phospho-Histone H3 (Thr3) Recombinant Rabbit mAb

db11513

Datasheet

Product Name : Phospho-Histone H3 (Thr3) Recombinant Rabbit mAb

Cat.No.: db11513

Synonyms : H3/A; H3FA

Application : WB, IHC-P, ICC/IF, FC

Reactivity : Human

Host species : Rabbit

Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
Immunogen A synthetic phosphopeptide corresponding to residues surrounding Thr3 of human Histone H3
Gene ID 8350
Swiss Prot P68431
Synonyms H3/A; H3FA
Reactivity Human
Application WB, IHC-P, ICC/IF, FC
Recommended dilution WB: 1:5000-1:50000
IHC-P: 1:100-1:200
ICC/IF: 1:100-1:200
FC: 1:100
Calculated MW 15 kDa
Observed MW 15 kDa
Host species Rabbit
Clonality Monoclonal
Isotype IgG
Purity Affinity Purification
Conjugation Un-conjugated
Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Western blot detection of Phospho-Histone H3 (Thr3) in C6,3T3,Hela cell lysates using Phospho-Histone H3 (Thr3) antibody(1:1000 diluted).
Immunofluorescent analysis of HeLa cells using db11513 antibody (green), and DAPI (blue).

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