BackgroundThis gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]
ImmunogenA synthetic peptide of human 14-3-3 alpha/beta
Storage/StabilityStore at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Western blot detection of 14-3-3 alpha/beta in Hela,A549,MCF-7,U251,C2C12 cell lysates using 14-3-3 alpha/beta antibody(1:1000 diluted).
Western blot detection of 14-3-3 alpha/beta in Rat brain, Rat lung tissue lysates using NQO1 antibody(1:1000 diluted)
Immunohistochemical analysis of paraffin-embedded human tonsil using db11474 antibody.