BackgroundThe Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015]
Storage/StabilityStore at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Western blot detection of PLK1 in Jurkat,C6 cell lysates using PLK1 antibody(1:1000 diluted).
Western blot analysis of extracts from A549 cells using db11470 at 1:1000.
Immunofluorescent analysis of HeLa cells using db11470 antibody (green), and DAPI (blue).