BackgroundThis gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]
Storage/StabilityStore at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Western blot detection of ATG3 in K562,Rat Brain,C6,3T3,Hela cell lysates using ATG3 antibody(1:1000 diluted).
Immunohistochemical analysis of paraffin-embedded human tonsil using db11469 antibody.
Immunofluorescent analysis of HeLa cells using db11469 antibody (green), and DAPI (blue).