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Phospho-Smad2 (Ser250) Recombinant Rabbit mAb
db11432
Datasheet
- Background The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]
- Immunogen A synthetic phosphopeptide corresponding to residues surrounding Ser250 of human Smad2
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Gene ID
4087
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Swiss Prot
Q15796
- Synonyms JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
- Reactivity Human, Mouse, Rat
- Application WB, FC
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Recommended dilution
WB: 1:1000
FC: 1:20 - Calculated MW 52,48 kDa
- Observed MW 58,62 kDa
- Host species Rabbit
- Clonality Monoclonal
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot detection of Smad2 (phospho-Ser250) in Hela,CHO-K1,C6 using Smad2 (phospho-Ser250) antibody(1:1000 diluted). -
Western blot analysis of extracts from Mouse kidney tissue using db11432 at 1:1000.
订购信息
- Package Price (RMB)
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
