您当前的位置：
BRG1 (DGR14531) Rabbit mAb
db11111
Datasheet
- Background Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity).
- Immunogen A synthetic peptide of mouse BRG1
-
Gene ID
20586
-
Swiss Prot
Q3TKT4
- Synonyms Brg1; SW1/SNF; HP1-BP72; SNF2beta; b2b692Clo; b2b508.1Clo
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, FC, IP
-
Recommended dilution
WB: 1:2000-1:20000
IHC-P: 1:100-1:200
ICC/IF: 1:200-1:500
FC: 1:100-1:200
IP: 1:10-1:100 - Calculated MW 185 kDa
- Observed MW 220 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR14531
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
-
Western blot detection of BRG1 in 293T,Hela,HepG2，A594 cell lysates using BRG1 antibody(1:1000 diluted).
订购信息
- Package Price (RMB)
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
