BackgroundThis gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008]
Storage/StabilityStore at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Western blot detection of Keap1 in Raw264.7 cell lysates using Keap1 antibody(1:1000 diluted).
Western blot analysis of extracts from Mouse liver tissue using db11088 at 1:1000.
Western blot analysis of extracts from HepG2 cells using db11088 at 1:1000.
Western blot detection of Keap1 in Hela, A549, C2C12 cell lysates using Keap1 antibody(1:1000 diluted).
Immunohistochemical analysis of paraffin-embedded human breast cancer using db11088 antibody.