Phospho-CDK1/2 (Thr14) Recombinant Rabbit mAb - diagbio | 戴格生物

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Phospho-CDK1/2 (Thr14) Recombinant Rabbit mAb

db11065

Datasheet

Product Name : Phospho-CDK1/2 (Thr14) Recombinant Rabbit mAb

Cat.No.: db11065

Synonyms : CDKN2; p33(CDK2)

Application : WB, IHC-P, IP

Reactivity : Human,Mouse,Rat

Host species : Rabbit

Background This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
Immunogen A synthetic phosphopeptide corresponding to residues surrounding Thr14 of human Cdk1/2
Gene ID 1017
Swiss Prot P06493
Synonyms CDKN2; p33(CDK2)
Reactivity Human,Mouse,Rat
Application WB, IHC-P, IP
Recommended dilution WB: 1:1000
IHC-P: 1:100-1:200
IP: 1:20-1:50
Calculated MW 34 kDa
Observed MW 34 kDa
Host species Rabbit
Clonality Monoclonal
Isotype IgG
Purity Affinity Purification
Conjugation Un-conjugated
Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Western blot detection of Cdk1/2 (phospho-Thr14) in HepG2,U2OS,U251 using Cdk1/2 (phospho-Thr14) antibody(1:1000 diluted)
Western blot analysis of extracts from HeLa cells using db11065 at 1:1000.
Immunohistochemical analysis of paraffin-embedded human lung cancer using db11065 antibody.

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