His Tag (2A2) Mouse mAb - diagbio | 戴格生物

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His Tag (2A2) Mouse mAb

db6491

Datasheet

Product Name : His Tag (2A2) Mouse mAb

Cat.No.: db6491

Synonyms : 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag

Application : WB, IP, ICC/IF

Reactivity : Transfected

Host species : Mouse

Background A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
Immunogen Synthetic peptide/HHHHHH conjugated to KLH
Synonyms 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag
Reactivity Transfected
Application WB, IP, ICC/IF
Recommended dilution WB: 1:1000-1:10000
IP: 1:20
ICC/IF: 1:100-1:500
Observed MW According to the fused protein
Host species Mouse
Clonality Monoclonal
Clonality No. 2A2-4H2-10D8
Isotype IgG1
Purity Affinity Purification
Conjugation Un-conjugated
Storage/Stability Store at -20°C. Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Stable for 12 months from date of receipt.

2ug His fusion protein+ Primary antibody dilution at 1) 1:5000. 2) 1:10000
Immunofluorescence analysis of His Tag (2A2) in 293 cells transfected with a Histag protein using His Tag (2A2) antibody(red),and DAPI (blue).
Immunoprecipitation analysis of Flag Tag (7E1) in 1、untransfected 293 cell lysates2、transfected 293 cell lysates with Histag fusion protein3、IP (untransfected 293+antiHis mAb+ Protein G agarose)4、IP (transfected 293+ normal mouse IgG+Protein G agarose)5、IP (transfected 293+antiHis mAb+ Protein G agarose)

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